Action of disodium ethylenediamine tetracetate on blood coagulation; evidence of the development of heparinoid activity during incubation or aeration of plasma.

T LIE ANTICOA(.ULANT PROPERTIES OF ethyleimediamine tetracetic acid were studied for the first time by 1)yckerhoff amid his co-workers.’ They comicluded that this compoumnd acted mainly as ami ant.ithrombin amid to a lesser degree as an antiprothrombin. Later Bosenfeld amid Jansky’ reported that disodiuim ethvlemiediamimie tetracetate or Ei)TA inhibits the tramisformatiomi of fibrinogen to fibrin and that. this inhibition can be reversed 1)y recalcificatiomi. Zucker’ observed that alt.houmgh EDTA prolongs the one stage prot.hromhin time, it does miot. affect the two stage test. and the labile factor is inhibited. This study was ummidertaken to ftmrther the understanding of the effect of EDTA upon blood coagulation.